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Old 12-10-2015, 09:23 AM   #173
KroSeq
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Location: Gothenburg

Join Date: Dec 2014
Posts: 4
Default SSRT IV vs SSRT II

Quote:
Originally Posted by Simone78 View Post
Hi,
I did exactly as described in the Nat Prot paper, just replacing SSRTII with SSRTIV and doing the RT for 15 min @ 50 degrees and inactivated the enzyme for 10 min @ 80 degrees.. I tested it only on tot RNA (10 pg) and I actually noticed that when I didnīt add betaine and MgCl2 I got worse results than when using them...the opposite of what you found. in my case the template switching went ok, at least based on the cDNA yield and size.
Best,
Simone
Hej Simone,
what is the benefit of using SSRT IV instead of SSRT II?
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