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Old 12-30-2015, 07:36 PM   #1
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
Default Single-cell RNA-seq with ERCC RNA Spike-In

Hi all,
I recently came across some single-cell rna-seq experiment, in which people always recommand using ERCC spike-ins for normalization. And I just wonder how much spike-ins should be added per cell, 1ul 1:5,000,000 diluted from original stock? And also how you guys keep these easy-degraded synthetic RNA on your hands? As super diluted RNA is not that stable even in -80C, I am afraid Also, there are two options, the regular one and the Exfold one, which one you guys prefer?

Wish you guys a happy new year

Gary

Last edited by kobeho24; 12-30-2015 at 07:39 PM.
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