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Old 12-31-2015, 05:22 AM   #3
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
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Quote:
Originally Posted by ostolop View Post
Hi!

This is a bit off-topic, and somewhat blatant self-advertising, but for what it's worth, we developed a method -- preparing it for publication, draft going on biorxiv soon -- to deal with single cell RNA-seq without ERCC spike-ins.

Here's some info on it http://genestack.com/single-cell-rnaseq/ and a bit more here https://genestack.com/blog/2014/09/2...ysis-tutorial/.

The method is available on Genestack.

--Misha
P.S. I'm the founder of Genestack, happy to answer questions etc.
Hi Misha,
From my point of view, technical noise in single cell experiment such as PCR bias should always be taken into account. As far as I know, ERCC and UMI are the only two options we have to defeat the technical noise to some degree. And I am not sure that the issue can be solved in a bioinformatic manner. Anyway, thank you for your reply. I will take a look at it and see if there is any excellent information for future reference.

Best,
Gary
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