Quote:
Originally Posted by ostolop
Hi!
This is a bit off-topic, and somewhat blatant self-advertising, but for what it's worth, we developed a method -- preparing it for publication, draft going on biorxiv soon -- to deal with single cell RNA-seq without ERCC spike-ins.
Here's some info on it http://genestack.com/single-cell-rnaseq/ and a bit more here https://genestack.com/blog/2014/09/2...ysis-tutorial/.
The method is available on Genestack.
--Misha
P.S. I'm the founder of Genestack, happy to answer questions etc. |
Hi Misha,
From my point of view, technical noise in single cell experiment such as PCR bias should always be taken into account. As far as I know, ERCC and UMI are the only two options we have to defeat the technical noise to some degree. And I am not sure that the issue can be solved in a bioinformatic manner. Anyway, thank you for your reply. I will take a look at it and see if there is any excellent information for future reference.
Best,
Gary