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Old 12-31-2015, 05:39 AM   #4
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Location: UK

Join Date: Nov 2011
Posts: 2
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Hi Gary,

You're absolutely right -- if you *have* spike-ins, it makes your experiment design more meaningful. We implemented methods that make use of spike-ins, of course, that goes without saying.

We ended up designing and implementing a non-parametric noise-reduction method because we got tasked with processing a dataset that didn't have spike-ins, ERCC or UMI...

We then tested our method on the Brennecke dataset and got high concordance! So, we know it works well..

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