SEQanswers - View Single Post - RNA-seq from single cells?

kobeho24 · 01-21-2016, 03:04 AM

Hi all,
Recently I’ve done 3 runs of single-cell RNA-seq (Smart seq2) trial. I just followed the original protocol. A highly reproducible background curve can be observed even in negative controls (only nuclease free water) when using our new SuperScript II RTase, which cannot be seen when using SSIII, or without RTase. Our bad SSII lot no. is 1688365.
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Does any of you guys have any recommendation of substitutive MMLV RTase? I saw ProtoScripts II & SS IV so far, any candidate else?

Best wishes!
Gary

01-21-2016, 03:04 AM	#178
kobeho24 Member Location: HKUST, Hong Kong Join Date: Apr 2015 Posts: 32	SSII contamination Hi all, Recently I’ve done 3 runs of single-cell RNA-seq (Smart seq2) trial. I just followed the original protocol. A highly reproducible background curve can be observed even in negative controls (only nuclease free water) when using our new SuperScript II RTase, which cannot be seen when using SSIII, or without RTase. Our bad SSII lot no. is 1688365. QQ??20160121185909.png QQ??20160121190101.png QQ??20160121190137.png Does any of you guys have any recommendation of substitutive MMLV RTase? I saw ProtoScripts II & SS IV so far, any candidate else? Best wishes! Gary Last edited by kobeho24; 01-21-2016 at 06:47 PM.