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Old 01-27-2016, 05:06 AM   #179
Simone78
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Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 208
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Quote:
Originally Posted by kobeho24 View Post
Hi all,
Recently Iíve done 3 runs of single-cell RNA-seq (Smart seq2) trial. I just followed the original protocol. A highly reproducible background curve can be observed even in negative controls (only nuclease free water) when using our new SuperScript II RTase, which cannot be seen when using SSIII, or without RTase. Our bad SSII lot no. is 1688365.
Attachment 4169

Attachment 4170

Attachment 4171

Does any of you guys have any recommendation of substitutive MMLV RTase? I saw ProtoScripts II & SS IV so far, any candidate else?

Best wishes!
Gary
I would use either SMARTscribe (Clontech) or Maxima H- (Thermo Scientific).
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