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Old 01-27-2016, 06:33 AM   #180
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
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Quote:
Originally Posted by Simone78 View Post
I would use either SMARTscribe (Clontech) or Maxima H- (Thermo Scientific).
Hi Simone,
Between these two, which one's better? It seems that SMARTscribe is similar to SSII and Maxima H- is similar to SSIV. Besides, as SMARTscribe is 100U/ul while Maxima H- is 200U/ul, do we still make it 100U in the 10ul RT reaction as it is in the Smart-seq2 protocol? Am I right?

Best!
Gary
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