SEQanswers - View Single Post - Allowing a high number of mismatches when mapping

Jane M · 07-26-2016, 02:31 AM

Thank you for your answer.

I am working on a sh screen. The first 22-30 bases are common to all sequences. Between 23 and 31 bases correspond to the sh in each sequence.
Since there is a problem of quality at the end (from the middle in fact), I use the minimum number of bases (from the left) needed to discriminate the sh.

07-26-2016, 02:31 AM	#5
Jane M Senior Member Location: Paris Join Date: Aug 2011 Posts: 239	Thank you for your answer. I am working on a sh screen. The first 22-30 bases are common to all sequences. Between 23 and 31 bases correspond to the sh in each sequence. Since there is a problem of quality at the end (from the middle in fact), I use the minimum number of bases (from the left) needed to discriminate the sh.