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Old 10-26-2016, 11:33 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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I've read that the Tn5 transposase stays bound to both ends of the DNA that it has cleaved. Perhaps the purification step removes both the protein (transposase) and the DNA it is attached to. Whereas treatment with SDS could denature the transposase, allowing the DNA to be recovered?

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Phillip
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