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Old 10-26-2016, 08:09 PM   #3
Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32

Originally Posted by pmiguel View Post
I've read that the Tn5 transposase stays bound to both ends of the DNA that it has cleaved. Perhaps the purification step removes both the protein (transposase) and the DNA it is attached to. Whereas treatment with SDS could denature the transposase, allowing the DNA to be recovered?

Hi Phillip,
Thanks for your reply. It may explain part of my confusion. But how come I cannot get a detectable mount of DNA with the condition of using SDS prior to beads purification? That doesn't make sense.
And the most important thing is that there isn't any SDS treatment but purification step in Nextera DNA prep kit, and it must work, otherwise illumina can't make it on the market.

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