Quote:
Originally Posted by pmiguel
I don't know. Does SDS prevent DNA from binding to Ampure beads, perhaps?
Nextera may have additives in its buffers, or even a mutated Tn5 transposase that performs differently.
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Phillip
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I have no idea about that either. But I already super diluted the reaction containing limited amount of SDS prior to beads purification~ I suppose it should be the buffer issue, somehow illumina just has tricks~
Gary