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Old 10-27-2016, 08:01 PM   #5
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
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Quote:
Originally Posted by pmiguel View Post
I don't know. Does SDS prevent DNA from binding to Ampure beads, perhaps?

Nextera may have additives in its buffers, or even a mutated Tn5 transposase that performs differently.

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Phillip
I have no idea about that either. But I already super diluted the reaction containing limited amount of SDS prior to beads purification~ I suppose it should be the buffer issue, somehow illumina just has tricks~

Gary
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