Originally posted by hydnazul
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potassium acetate was/is used just to replace the Tris-HCl as tagmentation buffer and it´s used at the same conc (10 mM for the 1x). After trying many different tagmentation buffers we noticed that there is no dramatic difference between TAPS, Tris-HCl, KOAc and HEPES, as long as the conc is 10 mM, the pH around 7.5-8.5 and you have 5 mM MgCl2 (1x).
Best,
Simone
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