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Old 12-13-2017, 03:26 AM   #1
Location: UK

Join Date: Jan 2014
Posts: 75
Default Maximum read cycles in NextSeq kits?

We are using the 75 cycle 400M kit for paired-end sequencing.
Our understanding is that there are 75 read cycles, plus another 16 cycles (2x8 cycles) available for barcode detection. Total of 91 cycles.
I know that it is possible to split the read cycles into a *minimum* of 2x37. However, our samples are only single-indexed and only use 6 bases for the index.
Thus, in theory, there are another 10-11 cycles of chemistry in these kits, suggesting that 2x42 bp should be possible - plus 6 cycles for indexing.
Does anyone have experience with setting up such a custom run?
Would we simply specify 2x42 bp in the sample sheet?
Many thanks!
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