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Old 08-22-2018, 08:35 AM   #8
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Location: Australia

Join Date: Aug 2016
Posts: 1
Default Spliced spike-in RNA standards (sequins)

Dear all,

Also a bit of self-advertising - but we have developed a new set of RNA spike in controls (we call them sequins) that can be used in single-cell RNA sequencing instead of ERCCs.

Unlike ERCCs, they are modeled on human genes, so they include spliced alternative isoforms. Additionally, they have been prepared with care to provide a tighter reference ladder, wider dynamic range, and no-thaw manufacture (to ensure full length transcripts).

They are free for non-profit research, and we can send as much as you need for your experiments. Order them from at

We also describe them in detail in the following Nature Methods paper:

Hope they help!
TimMercer is offline   Reply With Quote