SEQanswers - View Single Post - Single-cell RNA-seq with ERCC RNA Spike-In

TimMercer · 08-22-2018, 08:35 AM

Dear all,

Also a bit of self-advertising

- but we have developed a new set of RNA spike in controls (we call them sequins) that can be used in single-cell RNA sequencing instead of ERCCs.

Unlike ERCCs, they are modeled on human genes, so they include spliced alternative isoforms. Additionally, they have been prepared with care to provide a tighter reference ladder, wider dynamic range, and no-thaw manufacture (to ensure full length transcripts).

They are free for non-profit research, and we can send as much as you need for your experiments. Order them from at www.sequin.xyz/order

We also describe them in detail in the following Nature Methods paper: https://www.nature.com/articles/nmeth.3958

Hope they help!
Tim.

08-22-2018, 08:35 AM	#8
TimMercer Junior Member Location: Australia Join Date: Aug 2016 Posts: 1	Spliced spike-in RNA standards (sequins) Dear all, Also a bit of self-advertising - but we have developed a new set of RNA spike in controls (we call them sequins) that can be used in single-cell RNA sequencing instead of ERCCs. Unlike ERCCs, they are modeled on human genes, so they include spliced alternative isoforms. Additionally, they have been prepared with care to provide a tighter reference ladder, wider dynamic range, and no-thaw manufacture (to ensure full length transcripts). They are free for non-profit research, and we can send as much as you need for your experiments. Order them from at www.sequin.xyz/order We also describe them in detail in the following Nature Methods paper: https://www.nature.com/articles/nmeth.3958 Hope they help! Tim.