SEQanswers - View Single Post - PCR validation of ATAC-seq library

MzwaneleN · 10-09-2020, 03:07 PM

I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing banding pattern. Do you perhaps know what could cause this? Could this be inherent to an ATAC-seq library considering that the template is not just one length size but varying lengths?

10-09-2020, 03:07 PM	#1
MzwaneleN Junior Member Location: Ottwa Join Date: Jul 2020 Posts: 2	PCR validation of ATAC-seq library I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing banding pattern. Do you perhaps know what could cause this? Could this be inherent to an ATAC-seq library considering that the template is not just one length size but varying lengths? Last edited by MzwaneleN; 10-09-2020 at 03:12 PM.