SEQanswers - View Single Post - initial amount of total RNA or enriched small rna for small rna seq

Deli Çoban · 05-30-2011, 01:35 PM

First, thanks ishmael for your response

We want to sequence two endemic fish species whose genome are not sequenced and annotated in database. Our study mainly covers the microRNA sequencing and their proposed relatively lenght is between 18-30 nt. My question is how much (ng or ug) the initial RNA quantity (small enriched rna or total rna) should be?

thanks...

05-30-2011, 01:35 PM	#3
Deli Çoban Junior Member Location: evde Join Date: May 2011 Posts: 6	First, thanks ishmael for your response We want to sequence two endemic fish species whose genome are not sequenced and annotated in database. Our study mainly covers the microRNA sequencing and their proposed relatively lenght is between 18-30 nt. My question is how much (ng or ug) the initial RNA quantity (small enriched rna or total rna) should be? thanks...