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Old 05-31-2011, 10:39 PM   #4
Location: NY, US

Join Date: Jul 2008
Posts: 17

Originally Posted by Deli Çoban View Post
First, thanks ishmael for your response

We want to sequence two endemic fish species whose genome are not sequenced and annotated in database. Our study mainly covers the microRNA sequencing and their proposed relatively lenght is between 18-30 nt. My question is how much (ng or ug) the initial RNA quantity (small enriched rna or total rna) should be?

Sorry, I forget the topic is about library preparation
Guys in our wet lab said it is hard to say the mass of total RNAs, may be 1mg? They just followed the protocols. Sometimes only when the sequencing finished then you know if the exaction is messed up or not. In the beginning, some sequencing batches of our samples were full of adaptor sequences only, useless .
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