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Old 06-04-2011, 04:47 PM   #5
Deli Çoban
Junior Member
Location: evde

Join Date: May 2011
Posts: 6

Originally Posted by ishmael View Post
Sorry, I forget the topic is about library preparation
Guys in our wet lab said it is hard to say the mass of total RNAs, may be 1mg? They just followed the protocols. Sometimes only when the sequencing finished then you know if the exaction is messed up or not. In the beginning, some sequencing batches of our samples were full of adaptor sequences only, useless .
Hi, ishmael

During the library preparation, approximately 1 ug (< 50 pb) enriched small RNA sould be suffecient to get proper sequences for illumnia hiseq2000, according to friends who runs this machine routinely. But, now I embark on collecting part of article about my subject. And, I am planing to list this information here.

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