View Single Post
Old 04-22-2018, 12:18 AM   #1
soungalo
Junior Member
 
Location: Israel

Join Date: Apr 2018
Posts: 3
Cool 454 GS FLX Titanium 'paired end' protocol

Hi,
I know this is an obsolete technology, but I came across some data produced on the 454 instrument (archived in SRA/ENA) which I'd like to use for assembly.
According to the meta data, insert sizes are 8kb and 20kb, but I wasn't able to figure out the protocol used to produce the libraries. I gather that this is some variant on what we'd now call mate-pair, but I'm not sure if I can treat the data exactly the same way as data coming from Illumina mate-pair libraries, in terms of read orientation, insert size distribution etc.
Had anyone worked with such data, or can direct me to some documentation about it? I failed to find anything in the current Roche docs.
Thanks!
soungalo is offline   Reply With Quote