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Old 05-21-2019, 12:27 PM   #1
Junior Member
Location: Sheffield

Join Date: May 2019
Posts: 3
Default High RIN low DV200?? Can anybody shed some light?

I've recently sent off E. coli RNA samples for RNA-seq. The company we have used for sequencing have replied and said all samples failed QC due to degradation. I am looking at the Tapestation values and some of these samples have a RIN up to 9.5 but then a DV200 of 25. I believe this is due to the large distinct band at approximately 95 bp- (no smearing!) which I believed were tRNA. I am now unsure as to whether to proceed with sequencing as they cannot guarantee sequencing results. Has anyone had experience of such a contrast in RIN and DV200 numbers previously? And then gone on to successfully perform the sequencing?
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