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Old 06-03-2014, 04:35 AM   #5
Junior Member
Location: Antwerp

Join Date: Jun 2014
Posts: 2

Hi Jeff, do you have a trace of the final library that is actually put on the SMRT cell?
Might be that there simply are too many small fragments, which preferentially load on the machine.
It's a bit of an obvious thing, since a seq center that regularly uses PacBio should know this and size select to retain only the longest fragments. But you never know..
MatDeclercq is offline   Reply With Quote