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Old 05-15-2015, 02:11 AM   #1
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
Default Is a purification step between cDNA generation and library construction necessary?

Hi all,
Recently, I was wondering that is it necessary to perform a purification step after cDNA generation and prior to library construction? Because I am gonna do the cDNA generation in a very tiny volume. The library construction can be either ligation-based or tagmentation-based.
Best!
Gary
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