SEQanswers - View Single Post - Is a purification step between cDNA generation and library construction necessary?

nucacidhunter · 05-27-2015, 09:27 PM

I think by cleaning up cDNA uniformity and consistency among samples and batches will be increased. Non-cleaned cDNA would have various amounts of left over oligos and salts which may affect library quality.

05-27-2015, 09:27 PM	#5
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	I think by cleaning up cDNA uniformity and consistency among samples and batches will be increased. Non-cleaned cDNA would have various amounts of left over oligos and salts which may affect library quality.