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Old 02-20-2019, 07:09 PM   #3
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Originally Posted by nucacidhunter View Post
I assume you have done PCR wit ITS region specific primers with no Illumina overhang or full adapter sequences. In this case they must have prepared library by adapter ligation which will result in non-direction library so R1 and R2 can start for some fragments from forward direction and for others from reverse direction.

You should check some reads from R1 and R2 sequence folders and you should see in both sequences starting with forward and reverse primers, unless they have been sequenced with custom primers or have been trimmed to remove PCR primers.
I don't quite understand, but I didn't do PCR. I gave them gDNA. They did the PCR and sequencing for me.

They didn't give me raw fastq files such as R1 and R2. They only give me a combined fasta file. I only find adapter sequence and forward primer sequences in the fast file. I don't they if they removed reversed primers or they only did sequencing using forward primer.
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