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Old 07-22-2014, 01:08 PM   #1
Location: Missoula, MT

Join Date: Jan 2014
Posts: 19
Question Degraded gDNA & TruSeq Nano Kit

Hi All,

I am hoping to prepare libraries with Illumina's TruSeq Nano kit and although Illumina recommends only using high-quality genomic DNA, I am wondering if anyone has experience using degraded gDNA with this kit. Most of our samples are degraded to some extent, but some are high quality. I suspect that all or most of the DNA degradation occurred as a result of poor environmental conditions in the field prior to collection of muscle tissue from carcasses.

In hopes of keeping our sample size as high as possible, I'm wondering if it might be worth it to try to prepare libraries with the degraded DNA samples. Could I size select the samples to remove the smaller degraded fragments? I know that DNA damage is a considerable factor here, and want to avoid factoring that into data analysis further downstream...

Thanks! Replies are much appreciated!
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