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Old 07-23-2014, 05:06 PM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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After sampleprep we always measure the sample with the BioAnalyzer, but with low quality samples there might be problems with adapter-dimers in the final prep result (this is the reason we also dilute the adapter, but sometimes it doesn't help).
Presence of adapter in final library even after diluting adapters is a good indication that library preparation method has been less robust and only sub-nanograms of input has been converted into library, which is expected from low quality input.

Quote:
Any favorite or recommended DNA repair enzyme mixes?
I am only aware of NEB PreCR Repair Mix which repairs few common damages.
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