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Old 09-24-2014, 06:56 AM   #9
Location: Netherlands

Join Date: Jul 2013
Posts: 13

In my current protocol we share our gDNA with the covaris (duty cycle=10%,intensity=5.0, burst/sec=200, t=240sec, mode=frequency sweep) and we our getting good results. Without the shearing we also find that there are still very large fragments in the 'degrade' FFPE gDNA.

I have received a tip recently, but I have not tried it yet, where it was said that the shearing of the gDNA with the covaris throws the gDNA up into the covaris tube, thus leaving some long fragments to remain in the tube... recommendations: remove tube each minute and give it a short spin, thus making sure all the gDNA is fragmented.
Does anyone has any experience with this?
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