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Old 09-24-2014, 01:56 PM   #10
kzarn
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Location: Missoula, MT

Join Date: Jan 2014
Posts: 19
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WhiteSeal - I was originally trying to fragment 52Ál of DNA in the Covaris MicroTubes (snap-tops), and was worried about sample splashing up on the sides of the tubes during fragmentation. Our Genomics Core manager said that completely filling the tube with 132Ál of DNA sample will lead to more efficient and even fragmentation. I have been using 132Ál of DNA sample in my fragmentation protocol and have seen much narrower size ranges post fragmentation.

If you have enough DNA sample you might try to completely fill the tubes rather than try to fragment samples in partially filled tubes and deal with spinning them down midway through the fragmentation protocol.
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