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Old 07-15-2015, 09:58 AM   #2
jpmirandam
Junior Member
 
Location: Santiago, Chile

Join Date: Jan 2013
Posts: 4
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Dear cknox,

I bought mate pair kits, both for illumina and ion torrent in order to prepare 8 kb insert libraries.

None of the protocols has worked for us and we lost more than 98% of the DNA before adaptor ligation and purification using AMPureXP (Our AMPureXP work correctly for other protocols).

After size selection, almost 100% of remaining DNA is recovered, but not enough to continue with coupler ligation (lower than 150 ng).

I wonder if other researchers have reported these problems and if you have suggestions to go ahead with the protocol.

Best regards,

JP.
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