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Old 09-25-2015, 02:40 PM   #5
Location: US

Join Date: Aug 2014
Posts: 23

Sorry to dig up the old post, I think my question is relevant to this discussion.

From practical point of view, technical replicates from the same RNA sample can be merged together into a single fastq file for mapping and subsequent counting. Is this the correct approach?

Alternatively, should mapping be done separately and counts added subsequently? Tophat is my current mapper, but please commend on whether this works with other mappers. Thanks
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