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Old 07-19-2017, 10:34 PM   #2
huguesparri
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Location: Montpellier (France)

Join Date: May 2008
Posts: 93
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Hello,

This kind of protocol is typical of smallRNAseq.
It uses a truncated T4 RNA ligase 2 and a modified 3' adapter.
Trunc. T4 RNA ligase is unable to adenylate the 5' end of its substrate so it uses the modification of the 3' primer (rApp) to perform this step.
The second ligation uses a classical RNA ligase.

I wasn't aware of such protocols used for RNAseq...
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