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Old 11-03-2017, 03:26 PM   #5
jwfoley
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Location: Stanford

Join Date: Jun 2009
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Quote:
Originally Posted by luc View Post
Have you perhaps tried extending the UMI a bit?
No, I'm concerned that having a lot of random bases would invite mispriming and other molecular tomfoolery, plus it's a waste of sequencing cycles if you're using short reads, as we do. Oh, and template switching is sensitive to the length of the oligo. Better instead to use a deduplication algorithm that's robust against UMI collisions, but that's a subject for another paper...

Last edited by jwfoley; 11-03-2017 at 03:27 PM. Reason: added another thought
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