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Old 04-06-2020, 08:22 AM   #1
miseq_user
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Location: United States

Join Date: Apr 2020
Posts: 1
Question Library with strong secondary structures

What is known about the strand displacement activity of the DNA polymerase Illumina uses for bridge amplification? I have a library with strong secondary structures and I am worried that clusters do not form well due to the secondary structures. Does anyone have any tips to improve cluster formation for such a library?
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