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Old 03-09-2012, 11:32 PM   #4
fastidiousme
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Location: India

Join Date: Mar 2012
Posts: 10
Default Reduced Representation Library

Hi Dale,

Thank you for replying to my previous post. I wonder if you have an idea about constructing reduced representaion libraries (RRLs). I have been looking for methods to short list a meth-sensitive and a meth-insensitive restriction enzyme to be able to digest an entire plant genome and construct an RRL for the same.

I have read multiple research papers on how to construct RRLs but none of them mentions the ways to narrow down to one a suitable RE for whole genome digestion.

Well no pressure but it would be great if you could help in this regard! I am kind of desperate for answers here.

Thanks,

Shalini
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