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Old 04-25-2019, 06:40 AM   #1
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Join Date: Feb 2012
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Default Picard InsertSizeMetrics Output Interpretation


I am looking at the data from my pilot Miseq Nano run performed on ATAC-seq libraries.
I wanted to see whether the fragment size distribution pattern corresponds to the one normally observed in ATAC libraries (nucleosome-free regions followed by nucleosomes), and ran Picard InsertSizeMetrics. I'm struggling with the interpretation of its output: what's he difference between FR count and RF count? Why am I getting distributions that are so different? Please see the plots attached. Thanks!
Attached Images
File Type: png Insert size FR title.png (41.4 KB, 4 views)
File Type: png Insert size RF title.png (35.4 KB, 3 views)
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