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Old 08-06-2014, 11:54 PM   #10
younko
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Location: USA

Join Date: May 2014
Posts: 24
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Hello chadn737

I also have similar problems.
I do not have a replicate. but I have several patients having same disease.

For example, in my case, I have 10 patients having same disease.
We treated the drug and did RNA-seq for before-drug/after-drug.

What we want to do is to find the differentially expressed genes reponsing to the drug. Instead of replications, we have 10 patients samples.. for pre/post.

I used edgeR and DESeq for this.. but I could not find the any DEG with adjusted p value .. So I am thinking to look at the p value instead of adjusted pvalue by considering the fact that we can have FP... of course...

Would it be okay?
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