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Old 07-05-2016, 03:48 PM   #2
DStephens@NuGEN
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Location: SF Bay Area

Join Date: Jun 2016
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Hi sbchua.1990,
We have seen traces such as these when the chip priming/loading was not performed correctly. Did you ensure that the chip priming station plunger was set to the highest notch and the plunger was released after exactly 30 seconds? Was the chip loaded with fresh reagents?

A couple of other possibilities include overloading of the chip and salt or detergent carryover. You noted that you already tried diluting the samples; how much material was used? We typically load 1-5 ng/well onto the Pico chip.

Finally, have you tried an additional clean-up of your samples?

Hope that helps!
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Denise Stephens
Technical Support Scientist at NuGEN Technologies
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