We have used barcoded primers for a PCR and our aim is to pool 8 samples together before subjecting to library prep using True seq (a modified protocol for amplicons) so that we have 8 samples per index.
Can someone tell me what amount of DNA should be pooled from each sample/
The recommendation I know is conc > 50 ng/microl and amount > 10microg per index. So, for pooling per index, does each sample need to have >10 microg per it or the 10 microg can be made up from the different samples at equal amounts (say 2 microg each).
Thanks a lot
Can someone tell me what amount of DNA should be pooled from each sample/
The recommendation I know is conc > 50 ng/microl and amount > 10microg per index. So, for pooling per index, does each sample need to have >10 microg per it or the 10 microg can be made up from the different samples at equal amounts (say 2 microg each).
Thanks a lot
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