View Single Post
Old 11-20-2014, 09:13 AM   #2
Brian Bushnell
Super Moderator
 
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
Default

You should also consider running 2 PacBio SMRT-cells and redoing the assembly rather than generating LMP and scaffolding. We generally get microbes in 1 contig with 2 cells, which is not too expensive (~$200 each, plus some other costs). And with a 2.5Mbp genome, 2 cells is plenty.
Brian Bushnell is offline   Reply With Quote