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Old 06-14-2016, 04:11 PM   #1
Autotroph
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Location: Europe

Join Date: Oct 2010
Posts: 22
Default FPKM vs gene length

Hi,

I used the program RSEM to calculate the expression level of genes in my RNA-seq data (Illumina paired-end) from human cell lines.

Now i see that the FPKM and gene length are positively correlated ("kendall" rank) with a r of 0.5. Is this expected?

Can you please explain why the FPKM is correlated with gene length?

Thanks,
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