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Old 08-10-2017, 11:27 PM   #4
colindaven
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Location: Germany

Join Date: Oct 2008
Posts: 415
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Pooling is always problematic, whether simulation is done before or after or not.

I always try to dissuade experimentalists from pooling. There are so many biases in the data anyway. Pools are rarely if ever clean - i.e. derived from one phenotype - so a range of biological biases are there as well. Also, expression is highly divergent between individuals.

I am no GWAS expert, but would advise against advertising this as GWAS. Perhaps a followup tests using Sanger sequencing etc of PCRs amplicons from individual (non-pooled) samples from the most important identified regions might provide clarity as to whether this is a true phenomenon or artifact of the expt design ?
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