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Old 09-07-2017, 10:03 AM   #4
finswimmer
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Location: Europe

Join Date: Oct 2016
Posts: 59
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Hello,

are you sure you loaded 1.25nM? Normal final concentrations of the library on a MiSeq are between 8-16pM.

What was the concentration of the library at the end of the library preparation? How did you quantify?

What is the average fragment size? How did you measure it? Whith Bioanalyzer/Tapestation? Can we see the profile?

fin swimmer

Last edited by finswimmer; 09-07-2017 at 10:19 AM.
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