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Old 05-28-2019, 08:40 AM   #9
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Location: Arizona

Join Date: May 2019
Posts: 8

Originally Posted by itstrieu View Post
The only two things I can think of is DNA in buffers containing high concentrations of EDTA, other chelating agents or salts may inhibit the
end repair and A-tailing reaction. The other is something going wrong with library amplification (maybe your index primers).

If you can, check the concentration or run a gel/tape of the adapter-ligated library to see if the yield is reasonable going into library amplification.
Thank you for the great advice. I'll run a gel confirmation to see if my adapter ligated libraries still have sample.
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