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Old 05-13-2011, 05:16 PM   #21
SamH
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Location: Moscow, ID

Join Date: Sep 2010
Posts: 16
Default Isolated B's

Hi, hopefully I can revive this old thread for a little. I just got a big dataset from a HiSeq2000 machine at Berkeley, I'm not sure which version of the Illumina pipeline was used, but I do see single "B" qualities in some reads
i.e.


@HS1_0077:5:1101:1205:2082#0/1
NCCCCAAAGCATGATGTTTCCACCCCCATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGACCGATATCGTATGCCGTCTTCCGC
+HS1_0077:5:1101:1205:2082#0/1
BTSTTV[VYYc_ac_cccccccc[YUYV_cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@HS1_0077:5:1101:1231:2094#0/1
NTGTGGTATATATGCATGTAGTTACTTGGCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTCCACGATCTCCACACACACCCTCT
+HS1_0077:5:1101:1231:2094#0/1
BUPNUSSUUUcccac_c_ccccc_ccc_caBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB


SamH
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