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Old 05-14-2011, 12:38 AM   #22
maubp
Peter (Biopython etc)
 
Location: Dundee, Scotland, UK

Join Date: Jul 2009
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As discussed earlier in this thread, you can have lone B qualities (PHRED 2) in the quality string, and a trailing block of B markers as well. The second example here specifically copes this:
http://news.open-bio.org/news/2010/0...q2-trim-fastq/
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