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Old 03-09-2017, 10:41 PM   #105
alexhaj
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Location: North America

Join Date: Mar 2015
Posts: 6
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Good work Brian! I am skeptical about your claim that NextSeq has less crossover than HiSeq or Miseq however. Would you please provide some data to back this up. And if that is the case maybe it's simply because demultiplexing is being done by CASAVA v2 on NextSeq and CASAVA v1 on HiSeq and Miseq. What if you did demultiplexing yourself, taking into account Quality scores (which I assume is not typically done).
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