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Old 04-23-2010, 08:28 AM   #51
AdamB
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Location: uk

Join Date: Apr 2010
Posts: 43
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Does anyone know the primer concentrations required for SE ChIP library preparation?

This is the information given in the protocol:
Quote:
Prepare the following PCR reaction mix:
• DNA (36 μl)
• 5x Phusion* buffer (10 μl)
• dNTP mix (1.5 μl)
• PCR primer 1.1 (1 μl)
• PCR primer 2.1 (1 μl)
• Phusion* polymerase (0.5 μl)
The total volume should be 50 μl.
N.b. only 10 ng of DNA is used for the library preparation in the first place, so the primer concentrations might be lower than for whole genome DNA sequencing?

Thanks.

Last edited by AdamB; 04-23-2010 at 08:31 AM.
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