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Old 06-07-2011, 03:00 AM   #1
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Default ChIP-Seq: Single-tube linear DNA amplification (LinDA) for robust ChIP-seq.

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Single-tube linear DNA amplification (LinDA) for robust ChIP-seq.

Nat Methods. 2011 Jun 5;

Authors: Shankaranarayanan P, Mendoza-Parra MA, Walia M, Wang L, Li N, Trindade LM, Gronemeyer H

Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.

PMID: 21642965 [PubMed - as supplied by publisher]



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