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Old 09-28-2017, 01:58 AM   #1
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Location: India

Join Date: Sep 2017
Posts: 6
Default Why illumina index is read separately?


illumina has total 3 reads (see attached image):
1. Read1 > followed by removal/wash-off of Read1 product
2. Index read (read separately after binding of Index Primer downstream)
3. Read2

Any idea why Index can't be read continuously down as part of Read1? Has this something to do with the sequencing length limit of Read1 or some other technical constraint?

Thank you!
Attached Images
File Type: png PE-Read.PNG (10.9 KB, 39 views)
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