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Old 11-27-2013, 08:09 AM   #25
TonyBrooks
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Location: London

Join Date: Jun 2009
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You remove the second strand before PCR. This means the template for the reaction is the first strand only. In an unstranded assay, both strands are used as template - so library is created from both strands.
The PCR amplicon is not digested - it is double stranded DNA.
Look at the jpg in the link. Sequencing is always in the blue to red direction.
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